Culture of Cells for Tissue Engineering is a new volume in the John Wiley series Culture of Specialized Cells, with focus on procedures for obtaining, manipulating, and using cell sources for tissue engineering. The book has been designed to follow the successful tradition of other Wiley books from the same series, by selecting a limited number of diverse, important, and successful tissue engineering systems and providing both the general background and the detailed protocols for each tissue engineering system. It addresses a long-standing need to describe the procedures for cell sourcing and utilization for tissue engineering in one single book that combines key principles with detailed step-to-step procedures in a manner most useful to students, scientists, engineers, and clinicians. Examples are used to the maximum possible extent, and case studies are provided whenever appropriate. We first talked about the possible outline of this book in 2002, at the World Congress of in vitro Biology, encouraged by the keen interest of John Wiley and inspired by discussions with our colleagues.